Invitrogen™ IL-10 Human ELISA Kit

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Catalog Number	Mfr. No.	Quantity
ENEHIL102	Invitrogen™ EHIL102	2 x 96 Tests
ENEHIL105	Invitrogen™ EHIL105	5 x 96 Tests
ENEHIL10	Invitrogen™ EHIL10	96 Tests

Description

Assay IL-10 (human interleukin-10) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-10 ELISA Kits.

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human interleukin-10 (IL-10)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 15.4 to 600pg/mL

• Sensitivity: < 3pg/mL

• Precision: Intra-assay CV < 10%; inter-assay CV < 10%

• Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Accession Number: P22301
• Assay Sensitivity: <3 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Specificity: No significant cross-reaction with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CSIF,GVHDS,IL-10A,TGIF,IL10
• Intraassay CV: <10%
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 15.36-600 pg/mL
• Description: Human IL-10 ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Plasma, Serum, Supernatant, Urine
• Target Species: Human
• Gene ID (Entrez): 3586
• Interassay CV: <10%
• Label or Dye: HRP
• Precision: Intra-assay CV <10%; inter-assay CV <10%
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL
• Target: Human
• Total Assay Time: 3 hr.