PCR and qPCR
Products for Your Workflow
Polymerase chain reaction (PCR) and quantitative PCR (qPCR) are methods used to make copies of genetic material so that small amounts can be tested. Whether you are cloning DNA, conducting mutagenesis experiments, performing forensic or parental testing, or detecting pathogens, you can find the primers, polymerases, master mixes, dyes, and thermal cyclers you need to complete your workflow.
Sample Preparation
Preparing samples for PCR or qPCR typically requires extraction and purification of nuclear material. Depending on the sample source, this may involve mechanical manipulation via homogenization or require the use of resins or beads to help bind the nucleic acids prior to purification. Prepare your samples with the help of DNA and RNA extraction, isolation, and purification kits, plus reagents, buffers, and more.
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Featured Products
Catalog Number 45-001-810
Cytiva triplePrep Kit
Catalog Number 09-920-066
Cytiva Sera-Xtracta Cell-Free DNA Kit
Catalog Number 4530582
Thermo Scientific™ GeneJET Gel Extraction Kit
Resources
MP Biomedicals Lysing Matrix Flier
- Filename
- mp-biomedicals-lysing-matrix-flyer-new.pdf
- Size
- 338 KB
- Format
- application/pdf
PCR Solutions Brochure
- Filename
- fisher-scientific-pcr-solutions-brochure.pdf
- Size
- 6 MB
- Format
- application/pdf
RNA reference guide
- Filename
- rna-reference-guide.pdf
- Size
- 2 MB
- Format
- application/pdf
PCR Amplification
Amplification is performed by combining a DNA segment, polymerase, nucleotide solution, and primers in a PCR tube or microplate. A thermal cycler is used to perform the following steps, repeating the process 35 to 40 times.
Denaturation: Hydrogen bonds are broken, DNA separates
Annealing: Primers and polymerase bind to the DNA
Extension: Nucleotides pair with the DNA to form a new complementary strand
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Featured Products
Catalog Number F553L
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Catalog Number A48141
Applied Biosystems™ VeritiPro™ Thermal Cycler, 96-well
Catalog Number 4007188
Cytiva PuReTaq Ready-To-Go™ PCR Beads
qPCR Reverse Transcription
In real-time PCR, RNA is converted to cDNA and then amplified using PCR. Common real-time PCR applications detect expressed genes, examine transcript variants, and create cDNA templates for cloning and sequencing procedures. Find polymerases, oligonucleotides, master mixes, and more below to support your real-time PCR assays.
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Featured Products
Catalog Number 7184592
Invitrogen™ SuperScript™ IV VILO™ Master Mix
Catalog Number PRA5003
Promega GoScript™ Reverse Transcriptase
Catalog Number FERR0241
Thermo Scientific™ dNTP Mix (2 mM each)
Resources
Invitrogen™ SuperScript™ IV VILO Master Mix Brochure
- Filename
- invitrogen-superscript-iv-vilo-master-mix-brochure.pdf
- Size
- 1 MB
- Format
- application/pdf
Applied Biosystems RT-PCR Solutions Product Overview
- Filename
- applied-bio-rt-pcr-solutions-product-overview.pdf
- Size
- 2 MB
- Format
- application/pdf
qPCR Quantification
Real-time PCR is used to detect specific DNA sequences. This method uses fluorescent dyes or probes to determine the amount of amplified material in your sample. Find a variety of master mixes, plus real-time PCR instruments, assays, and kits for quantifying your qPCR results.
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Featured Products
Catalog Number A28136
Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System, 96-well, 0.1 mL
Catalog Number 7180446
Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR
Catalog Number 44-187-16
Applied Biosystems™ TaqMan™ Array, Human Angiogenesis, Fast 96-well
Resources
Applied Biosystems Digital PCR System Guide
- Filename
- applied-bio-digital-pcr-guide.pdf
- Size
- 1 MB
- Format
- application/pdf
Applied Biosystems Digital PCR System Data Sheet
- Filename
- applied-bio-digital-pcr-data-sheet.pdf
- Size
- 193 KB
- Format
- application/pdf
Essential Supplies
Find HEPA-filtered PCR enclosures to help keep contaminating particulates out of your workspace. Shop single-use PCR tubes and tube strips, nucleic acid microplates, PCR enclosures, and other labware that is certified free of DNAse, RNAse, DNA, and other potential interferants.
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Featured Products
Catalog Number 3733177
Fisherbrand™ PCR Workstation
Catalog Number 3599534
Fisherbrand™ 0.2mL PCR Tubes
Catalog Number AB2396
Thermo Scientific™ Armadillo PCR Plate, 96-well, clear, clear wells
Resources
Corning™ Axygen™ PCR Microplate Data Sheet
- Filename
- corning-axygen-pcr-microplate-data-sheet.pdf
- Size
- 287 KB
- Format
- application/pdf
Fisherbrand PCR Workstation Data Sheet
- Filename
- fisherbrand-pcr-workstation-data-sheet.pdf
- Size
- 497 KB
- Format
- application/pdf