Molecular Biology Methods

Products for Your Workflow

Molecular biology techniques are used to investigate the structure and function of the cells and tissues of living organisms. These techniques may include DNA and RNA analysis, gene expression, biochemical analysis, and protein production. Find DNA and RNA testing products, including reagents for locating specific DNA sequences, as well as products for transfection, chemical and electroporation transformations, nucleic acid labeling and detection, and more.

Isolation & Purification

Well-prepared DNA is critical to your experiments and research, so how you isolate and purify your samples is important. Multiple methods exist for disrupting the cells, removing debris, capturing the DNA, removing contaminants, and eluting the DNA for use in downstream applications. Find products for isolation, extraction, purification, and more to complete this step of your process.

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RNA Isolation and Purification

DNA Extraction and Purification

Nucleic Acid Purification Systems

Resources

MP Biomedicals Life Sciences Research Flyer

Filename: mp-biomedicals-life-science-research-flyer.pdf
Size: 761 KB
Format: application/pdf

KingFisher Apex Instrument Brochure

Filename: thermo-scientific-kingfisher-apex-brochure.pdf
Size: 3 MB
Format: application/pdf

Invitrogen DNA Purification and Analysis Brochure

Filename: invitrogen-purification-analysis-brochure.pdf
Size: 13 MB
Format: application/pdf

Labeling, Detection & More

Tags or labels, called probes, are added to nucleic acids to enable detection or purification. Labels may include radioactive phosphates, biotin, fluorophores, and enzymes. Fluorescent in situ hybridization (FISH) is used to identify specific DNA sequences and tests can also be performed for pathogen detection. Find labeling and detection kits, spectrophotometers, fluorometers, and more below for your nucleic acid work.

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Nucleic Acid Labeling and Detection Kits

UV-Vis Spectrophotometers

Fluorometers and Fluorescence Polarization Systems

Resources

Thermo Scientific NanoDrop One Selection Guide

Filename: thermo-scientific-nanodrop-one-brochure.pdf
Size: 3 MB
Format: application/pdf

MP Biomedicals Sample Preparation Brochure

Filename: mp-biomedicals-sample-preparation.pdf
Size: 2 MB
Format: application/pdf

Thermo Scientific Security Software Flyer

Filename: thermo-scientific-software-flyer.pdf
Size: 452 KB
Format: application/pdf

Detecting Phenol in Nucleic Acid Samples

Amplification

The goal of PCR is the amplification of existing DNA. Ultimately, having multiple copies makes it possible to perform your assays. Choose from a variety of thermal cyclers, polymerases, master mixes, and other PCR systems to support your PCR amplification needs.

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Thermal Cyclers

DNA Polymerases

PCR Supplies

Resources

Fisher Scientific PCR Workflow Brochure

Filename: fisher-scientific-pcr-workflow-brochure.pdf
Size: 1 MB
Format: application/pdf

PCR Solutions Brochure

Filename: pcr-plastics-solutions-brochure-0619.pdf
Size: 3 MB
Format: application/pdf

Thermo Scientific Molecular Biology Handbook

Filename: thermo-scientific-molecular-biology-handbook.pdf
Size: 24 MB
Format: application/pdf

Cloning & Modification

Cloning and genetic modification are commonly used to treat genetic defects. The field of genetics can help with DNA manipulation in vivo, altering genes to correct mutations, introduce new genetic information, or remove specific DNA sequences. Shop enzymes and inhibitors, cloning kits, competent cells, and more to help you conduct your cloning and modification research.

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Enzymes and Inhibitors

PCR Cloning Kits

Competent Cells

Resources

Thermo Scientific Molecular Cloning Brochure

Filename: thermo-scientific-molecular-cloning-brochure.pdf
Size: 26 MB
Format: application/pdf

CRISPR-Cas9 Gene Editing Products Brochure

Filename: biotech-crispr-bro-22-648-0288.pdf
Size: 6 MB
Format: application/pdf

Transfection

Mimicking natural viral processes, transfection artificially introduces nucleic acids to cells, producing various chemical, biological, or physical changes. Although the term transfection was originally used to describe the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, the term currently refers to any artificial introduction of foreign nucleic acid into a cell. Search for transfection reagents, electroporation supplies, and more to help effectively conduct your transfection procedures.

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Transfection Reagents

Electroporators

Electroporation Cuvets

Resources

Mirus Bio Transfection Experts Brochure

Filename: mirus-bio-transfection-experts-brochure.pdf
Size: 1 MB
Format: application/pdf

Transfection Reagents and Electroporation Brochure

Filename: transfection-reagents-electroporation-brochure.pdf
Size: 1 MB
Format: application/pdf

Selecting the Best Transfection Method Article

Analysis

The yield from your PCR and molecular biology procedures must be further analyzed and evaluated to confirm the outcome of your assays, especially if the material is intended for other downstream applications. Shop electrophoresis equipment, gel stains, imaging and documentation systems, and more to help ensure the success of your molecular biology assays.