Nucleic Acid Labeling and Detection
Nucleic Acid Labeling and Detection
Nucleic acid labeling and detection products are used to add tags or labels to nucleic acids to enable detection or purification. The tagged nucleic acids are referred to as 'probes.'
Nucleotide labeling may include radioactive phosphates, biotin, fluorophores, and various enzymes. Bioconjugation methods that produce nucleic acid probes can also be adapted to attach them to other molecules (for targeted delivery) and surfaces (to facilitate immobilization).
Although nucleic acid probes can be labeled during synthesis, custom oligonucleotide probes can be expensive so many researchers choose to label their own.
Quick and efficient benchtop oligonucleotide labeling can be performed for making smaller probe amounts or when multiple probes with the same label are needed. Enzymatic methods are affordable for small-scale probe generation; chemical methods are more suited to larger scale reactions.
Probes can be labeled at either end of the oligonucleotide or randomly incorporated throughout the sequence. The choice of method depends on the degree of labeling required and whether changes will prevent theexpected interactions. Nucleic acid hybridization reactions can benefit from random label incorporation; protein interactions may require end-labelling.
Summary of Methods
Enzymatic
- TdT: ssDNA
- T4 RNA Ligase: ssDNA, RNA
- T4 PNK: ssDNA, RNA
- DNA Polymerase: DNA, RNA
- RNA Polymerase: RNA
Chemical
- Periodate: RNA
- EDC: DNA, RNA
- Nonspecific crosslinkers: DNA, RNA
Detection methods are biotin-based, chemiluminescent, fluorescent, radioactive, or the combination of several techniques. Tests may include nucleic acid tests for pathogen detection and fluorescent in situ hybridization (FISH) for specific DNA sequences in chromosomes.
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Filtered Search Results
Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Green Features | Fewer resources used, Less waste |
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Product Line | Qubit |
Invitrogen™ Quant-iT™ 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
For easy and accurate dsDNA quantitation, the Quant-iT 1X dsDNA HS and BR Assays enable up to 1,000 fluorescence-based assays in a 96-well microplate format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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For Use With (Equipment) | Microplate Reader |
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Product Line | Quant-iT |
Detection Method | Fluorescence |
Invitrogen™ Qubit™ dsDNA Quantification Assay Kits
Achieve accurate and precise quantification of double-stranded DNA with Qubit dsDNA HS and BR Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish dsDNA from ssDNA, RNA, protein, and free nucleotides.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
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Excitation/Emission | 510/527 |
For Use With (Equipment) | Qubit Fluorometer |
Product Line | Qubit |
Detection Method | Fluorescence |
Invitrogen™ Qubit™ RNA High Sensitivity (HS), Broad Range (BR), and Extended Range (XR) Assay Kits
Kits enable quick and sensitive detection of low and high abundance RNA, and can distinguish between RNA and DNA, protein, and free nucleotides.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Detection Method | Fluorescence |
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Invitrogen™ Qubit and Quant-it Assay Kit Standards
Achieve precise quantitation of dsDNA or RNA samples with stand-alone Qubit nucleic acid standards.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Product Line | Qubit |
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Invitrogen™ Quant-iT™ RNA Assay Kits and Quant-iT RNA HS Reagent
Achieve highly selective and accurate quantification of high-abundance RNA samples with the fluorescent Quant-iT RNA Reagent and Assay Kits. These kits are highly selective for RNA over double-stranded DNA, and provide sufficient material for at least 2000 assays in a 96-well microplate format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Product Line | Quant-iT |
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Detection Method | Fluorescence |
Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
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Excitation/Emission | 500/525 |
No. of Reactions | 200 Assays (2 mL assay volume) |
Quantitation Range | 50 pg to 2 μg |
For Use With (Equipment) | Microplate Reader |
Product Line | PICOGREEN, Quant-iT |
Detection Method | Fluorescence |
Invitrogen™ Quant-it™ RiboGreen RNA Assay Kit and RiboGreen RNA Reagent, RediPlate™ 96 RiboGreen™ RNA Quantitation Kit
Rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assays are enabled with the RediPlate 96 RiboGreen RNA Quantitation Kit. The microplate is pre-loaded with RiboGreen Reagent, which produces bright green fluorescence upon binding to RNA or DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Excitation/Emission | 500/525 |
For Use With (Equipment) | Microplate Reader |
Detection Method | Fluorescence |
Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.