Protein Biology Methods
Products for Your Workflow
Genes direct the production of proteins that play a significant role in diseases such as cancer, Alzheimer’s, and diabetes. Protein biology helps us understand the output of these genes and the way they influence our health. Through protein isolation, purification, separation, and detection, protein biology provides earlier and more accurate diagnoses.
1. Isolation
Isolate proteins from your mixture with the products below. This protein biology method will help you start your process and prepare you for future workflow steps. Find protein extraction essentials, dissociation reagents, and cell scrapers, plus additional products and resources.
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Featured Products
Catalog Number 45-001-810
Cytiva triplePrep Kit
Catalog Number 71-009-4MI
MilliporeSigma™ Novagen™ CytoBuster™ Protein Extraction Reagent
Catalog Number PI90078
Thermo Scientific™ B-PER™ with Enzymes Bacterial Protein Extraction Kit
Resources
MilliporeSigma™ BugBuster™ Master Mix Flyer
- Filename
- milliporesigma-bug-buster-master-mix-flier.pdf
- Size
- 277 KB
- Format
- application/pdf
2. Purification
Purification is another necessary protein biology method and involves cell lysis, protein binding, washing, and elution. The protocol used will depend on the protein you’re purifying. To get started, shop prepacked columns, products for protein purification, dialysis and desalting, and more. For further information, download our resources.
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Catalog Number PI88845
Thermo Scientific™ Pierce™ Protein A Magnetic Beads
Catalog Number 45-000-323
Cytiva HisTrap™ HP Prepacked Columns
Catalog Number PI89877
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Resources
Thermo Scientific Zeba Desalting Products Brochure
- Filename
- thermo-scientific-zeba-desalting-products-brochure.pdf
- Size
- 4 MB
- Format
- application/pdf
Thermo Scientific Protein Clean-Up Technical Handbook
- Filename
- thermo-scientific-protein-clean-up-technical-handbook-bn0322162.pdf
- Size
- 4 MB
- Format
- application/pdf
Thermo Scientific and Invitrogen™ Protein Preparation Handbook
- Filename
- thermo-scientific-and-invitrogen-protein-preparation-handbook-bn0526161.pdf
- Size
- 5 MB
- Format
- application/pdf
R&D Systems Recombinant Protein Brochure
- Filename
- rd-systems-recombinant-proteins-brochure-bn03301711.pdf
- Size
- 5 MB
- Format
- application/pdf
3. Separation
Solubility, size, charge, and other protein characteristics are used to separate proteins. These techniques include various forms of chromatography in addition to electrophoresis and dialysis. Find the products you need for each of these methods and more by browsing gels, columns, and reagents. Learn more by downloading our relevant resources.
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Featured Products
Catalog Number XP04205BOX
Invitrogen™ Novex™ Tris-Glycine Mini Protein Gels, 4–20%, 1.0 mm, WedgeWell™ format
Catalog Number PI26619
Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
Catalog Number 45-002-071
Cytiva HiPrep™ Q Fast Flow 16/10 Column
Resources
Thermo Scientific and Invitrogen Protein Gels Welcome Packs Brochure
- Filename
- invitrogen-protein-gel-welcome-packs-brochure-bn20170438.pdf
- Size
- 219 KB
- Format
- application/pdf
4. Detection
Western blotting and ELISA techniques are frequently used for protein detection. Fluorescence microscopy is also common. Browse related reagents, substrates, and imaging systems to help advance your protein detection protocol. For answers that inform your workflow, download our resources.
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Catalog Number PI34579
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Catalog Number IPVH08100
MilliporeSigma™ Immobilon™-P PVDF Membrane
Catalog Number A44240
Invitrogen™ iBright™ CL1500 Imaging System
Resources
Thermo Scientific and Invitrogen Western Workflow Solutions Brochure
- Filename
- thermo-scientific-invitrogen-western-workflow-solutions-brochure-bn0916161.pdf
- Size
- 12 MB
- Format
- application/pdf
Thermo Scientific HRP Chemiluminescent Substrates Flyer
- Filename
- thermo-scientific-hrp-chemiluminescent-substrates-brochure-bn1031161.pdf
- Size
- 995 KB
- Format
- application/pdf