Invitrogen™ IL-10 Human ELISA Kit

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Supplier:  Invitrogen™ EHIL102

Catalog No. ENEHIL102

Description

Assay IL-10 (human interleukin-10) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-10 ELISA Kits.

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

Specifications

• Accession Number: P22301
• Assay Sensitivity: <3 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Specificity: No significant cross-reaction with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
• Target Species: Human
• Gene ID (Entrez): 3586
• Interassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, HRP Dilution Buffer, 2 x 14mL
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 2 x 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL
• Target: Human
• Total Assay Time: 3 hr.

• Assay Range: 15.36-600 pg/mL
• Description: Human IL-10 ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Plasma, Serum, Supernatant, Urine
• Sufficient For: 2 x 96 microplate wells
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CSIF,GVHDS,IL-10A,TGIF,IL10
• Intraassay CV: <10%
• Label or Dye: HRP
• Precision: Intra-assay CV <10%; inter-assay CV <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.