cDNA Synthesis Kits and Master Mixes
cDNA Synthesis Kits and Master Mixes
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Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 5X |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Master Mix |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | High |
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Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Green Features | Less Hazardous, Less Waste |
For Use With (Application) | RT-PCR,Real Time PCR (qPCR) |
Format | Kit |
Reaction Format | Premixed Components |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | Cells |
Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | High |
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Format | Master Mix |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Concentration | 4X |
Technique | Template Switching Reverse Transcription |
Starting Material | Cells or RNA |
Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents
The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit
The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Polymerase | Platinum SuperFi U DNA Polymerase |
GC-Rich PCR Performance | High |
Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 10 kb |
Final Product Type | PCR Amplified cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription, Preamplification |
Starting Material | Cells or RNA |
For Use With (Application) | Next-Generation Sequencing (NGS),Real Time PCR (qPCR) |