Gibco™ TrypLE™ Select Enzyme (1X), no phenol red

Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.

Catalog Number	Mfr. No.	Quantity
50-591-419	Gibco™ 12563011	100 mL
50-591-420	Gibco™ 12563029	500 mL

Description

Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, TrypLE Select is:

• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective

This TrypLE Select is modified as follows:

With
• EDTA

Without
• Phenol Red

Gentle on cells
TrypLE Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE Select is room-temperature stable and ready to use. TrypLE Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE Select is free of animal-derived components and is formulated on dedicated animal origin-free equipment.

Product Intended Use
Customers using TrypLE Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
TrypLE Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Chelators: EDTA
• Cell Type: Mammalian Cells
• Concentration: 1 X
• Form: Liquid
• Without Additives: No Phenol Red
• Format: Bottle(s)
• Osmolality: 280 - 300 mOsm/kg
• Product Line: Gibco™
• Shipping Condition: Room Temperature
• Tested For: In vitro Bioassay

• Content And Storage: Storage conditions: 15–30°C, protect from light.
  Alternate storage conditions: 2–8°C or -5 to -20°C.
  Shipping conditions: Ambient.
  Shelf life: 24 months from date of manufacture.
• Classification: Animal Origin-free
• Culture Type: Adherent Cell Culture
• Product Type: Cell Culture Dissociation Reagent
• Endotoxin Level: Low
• Green Features: Energy efficient, Sustainable packaging
• pH: 7 to 7.4
• Remarks: Room Temperature Stable
• Source: Animal origin-free

Safety and Handling

ShelfLife : 24 Months

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.