Molecular Probes™ Propidium Iodide - 1.0 mg/mL Solution in Water

Propidium Iodide - 1.0 mg/mL Solution in Water

Supplier:  Molecular Probes™ P3566

Catalog No. P3566

Description

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ∼30–40 nm to the red and the fluorescence emission maximum is shifted ∼15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

Specifications

• Content And Storage: Contains 1 bottle of propidium iodide(1.0 mg/mL solution in water).
  Store in refrigerator (2–8°C) and protect from light.
• For Use With (Application): Viability Assay
• Product Type: Propidium Iodide
• Emission: 533⁄617
• Quantity: 10 mL
• Sub Cellular Localization: Cytoplasm & Cytosol

• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Dye Type: propidium iodide
• Form: Solution
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.