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Invitrogen™ Dextran, Texas Red™, 10,000 MW, Neutral
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants
Supplier: Invitrogen™ D1828
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Description
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.
- Succinimidyl coupling of dyes to dextran molecule, which, in most cases, results in a neutral or anionic dextran
- Suitable for neuronal tracing (anterograde and retrograde) in live cells
- Cell lineage tracing in live cells
- Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
- Investigating vascular permeability and blood-brain barrier integrity
- Monitoring acidification (some dextran-dye conjugates are pH-sensitive)
- Studying hydrodynamic properties of cytoplasmic matrix
- Most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red dyes are essentially neutral
Cell Analysis, Cell Tracing and Tracking, General Cell Tracing, Neuronal Tracing
Order Info
Shipping Condition: Room temperature
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Specifications
Dextran | |
Store in freezer (-5 to -30°C) and protect from light. | |
Room Temperature | |
Texas Red™ |
25 mg | |
Classic Dyes | |
595/615 nm |
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For Research Use Only. Not for use in diagnostic procedures.