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Invitrogen™ Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay
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Non-radioactive technique

Supplier:  Invitrogen™ C10289

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Catalog No. C10289


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Includes: The kit contains sufficient reagents for performing two plates in a 96-well plate format.

Description

Description

The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or 'click' reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor 488 alkyne. The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min/Max format.

  • Less hazardous

Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Blue, Green
Fluorescent
Fluorescence Microscope, High Content Analysis Instrument
Alexa Fluor™ 488, Hoechst 33258
96-well plate
Alexa Fluor™, Click-iT™, Molecular Probes™
Room Temperature
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
  • Store at ≤-20°C, desiccate and protect from light.
    		•
    High-content Screening (HCS)
    HCS Assay
    UV, Visible
    Less hazardous
    2 plates
    SDS
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    For Research Use Only. Not for use in diagnostic procedures.