Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
MP Biomedicals™ Lysozyme, MP Biomedicals™
Used for lysing E. coli and Streptomycetes for extraction purposes. Lysozyme, MP Biomedicals preferentially hydrolyzes the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. Enzyme activity: ≥20,000u/mg solid.
Supplier: MP Biomedicals™ 02100831.5
Description
An enzyme used in a variety of biochemical applications.
- For lysing E. coli and Streptomycetes for extraction purposes, such as extracting group specific antigen
- May act as a germinative agent of bacterial spores
- In humans, may be the mediator in the anti-tumor function of macrophages which secrete the enzyme
- There is evidence that cartilage lysozyme has a role in cartilage calcification
- The enzyme breaks down the cell walls of bacteria
- Used to prepare spheroplasts
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.
- These links occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.
- Gram-positive cells are quite susceptible to this hydrolysis, as their cell walls have a high proportion of peptidoglycan.
- Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
- The enzyme is active over a broad pH range (6.0–9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02–0.100M) than at pH 9.2 (0.01–0.06M).
Specifications
Greater than or equal to 20,000 U/mg | |
Lysozyme | |
2235.597 g/mol | |
Store at -20°C | |
Powder | |
500 mg |
White | |
9001-63-2 | |
Muramidase; Mucopeptide N-acetylmuramoylhydrolase | |
Chicken Egg White | |
Cell Lysis |
Safety and Handling
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?
Product Content Correction