Molecular Probes™ LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation

The LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.

Catalog Number	Mfr. No.	Quantity
L23105	Molecular Probes™ L23105	200 Assays
50-112-1524	Molecular Probes™ L34961	80 Assays
L34962	Molecular Probes™ L34962	400 Assays

Description

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation
• Kit has been optimized and validated for use with a UV laser flow cytometer
• Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time
• Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated
• If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles
• LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs

Optimal brightness

• The LIVE/DEAD™ Fixable Blue Stain was selected based on its fluorescent properties to provide a bright signal when excited with a UV laser
• The blue-fluorescent reactive dye has an excitation maximum of ∼350 nm, making it ideal for use with an UV laser, and an emission of ∼450 nm
• By switching viability staining from a channel off of the blue 488 nm laser to the UV laser, a channel is freed up on the flow cytometer for other reagents that are difficult to find in colors other than FITC or PE
• By using the UV laser, there is no spectral overlap issues with other common dyes, thus eliminating the need to compensate the viability stains from other stains in the panel
• Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments

Specifications

• Cell Permeability: Impermeant
• Color: Blue
• Detection Method: Fluorescence
• For Use With (Equipment): Flow Cytometer
• Dye Type: LIVE/DEAD™ Fixable Blue Dead Cell Stain
• Excitation Wavelength Range: 350 nm
• Format: Tube(s)
• Shipping Condition: Room Temperature

• Cell Type: Eukaryotic Cells
• Description: LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation
• For Use With (Application): Viability Assay
• Product Type: Dead Cell Stain Kit
• Emission: 450 nm
• Form: Solid
• Product Line: LIVE/DEAD™
• Solubility: DMSO (Dimethylsulfoxide)

For Research Use Only. Not for use in diagnostic procedures.