Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

Supplier:  Invitrogen™ K283020

Catalog No. K283020

Includes: Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Description

• Unique technology to minimize background
• Get your clone: 95% efficiency
• Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
• Kanamycin and Zeocin resistance genes for your choice of selection
• Convenient, validated restriction sites in MCS for further subcloning
• Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time

Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA

Specifications

• Bacterial or Yeast Strain: Mach1™
• Content And Storage: Box 1:
  • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Sterile water
  
  Store at -5 to -30°C.
  All reagents are stable for 6 months when properly stored.
  
  Box 2:
  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid
  
  Store at -68 to -85°C.
• For Use With (Application): Chromatin Biology
• Product Type: PCR Cloning Kit
• Vector: Blunt DNA Cloning Vectors

• Cloning Method: Blunt TOPO™
• Format: Kit
• Product Line: One Shot™
• Quantity: 25 Reactions

For Research Use Only. Not for use in diagnostic procedures.