Thermo Scientific™ Hypersil™ PREP HS C8 HPLC Columns

Use these preparative columns for scale-up purification with high phase loading. They are suitable for all USP-L7 applications.

Supplier:  Thermo Scientific™ 41105059270A

Catalog No. 03-253-016

Description

Get maximum loading for your scale up from analytical to a prep LC method with Thermo Scientific™ Hypersil™ PREP HS C8 reversed phase preparative HPLC column. With similar selectivity to C18 columns and predictable elution order, C8 columns are ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations. By providing high surface area particles with a 100Å pore size, and a variety of dimensions including an option for XtendedLife hardware, which improves column lifetime by maintaining a uniform packed bed.

Similar selectivity, less retention than C18 columns

• Successful retention and separation of moderately hydrophobic analytes.
• Ideal for fatty acids, which are too hydrophobic for analysis using C18 columns.
• C8 (USP L7) selectivity - high surface area particle with 100Å pore size.
• pH stable from 2 to 8.
• Maximum temperature of 60°C.

Faster separations

• Higher analytical throughput compared to C18 alkyl chain chemistry.
• Quicker compound elution due to reduced hydrophobic interactions.

Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.

Standard Threaded and Standard Flange Hardware

• Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.
• Standard threaded hardware for column i.d. < 40mm.
• Standard flange hardware for column i.d. ≥ 40mm.
• Standard hardware can be modified on request to be compatible with SFC.

XtendedLife Hardware

• Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
• Compatible with SFC and is available in dimensions up to 50mm i.d.

Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.

Customer Specific Orders

• If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements.
• Please contact your local sales representative for Customer Specific Orders.
• Custom built columns are not eligible for returns.

Specifications

• Column Format: Preparative Column, Standard
• Length (Metric): 50 mm
• Particle Size: 5 μm
• Pore Size: 100 Å
• Temperature: 60°C
• Stationary Phase: C8
• Carbon Load: 7%
• Endcapped: Yes

• Diameter (Metric): 20 mm
• Max. Pressure: 4300 psi (295 bar)
• pH: 2 to 8
• Surface Area: 350 m²/g
• USP Type: L7
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Column Type: Reversed Phase
• Phase: Reversed Phase