Cytiva HiTrap™ MabSelect™ Prepacked Columns

Use these columns in small-scale MAb purification in a continuous bioprocess. Cytiva Life Sciences™ HiTrap™ MabSelect™ Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes

Catalog Number	Mfr. No.	Flow Rate	Quantity	Diameter (Metric) Inner Column	Binding Capacity
45-001-463	Cytiva 28408255	<20mL/min.	Each	1.6 cm	∼30 mg/mL Human lgG at 2.4 min. residence time
45-001-465	Cytiva 28408258	<4 mL/min.	5 Pk.	0.7 cm	∼40 mg/mL Human lgG at 2.4 min. residence time
45-001-467	Cytiva 28408261	<4 mL/min.	5 Pk.	1.6 cm	∼40 mg/mL Human lgG at 2.4 min. residence time
45-001-464	Cytiva 28408256	<20mL/min.	5 Pk.	1.6 cm	∼30 mg/mL Human lgG at 2.4 min. residence time
45-001-462	Cytiva 28408253	<4 mL/min.	5 Pk.	0.7 cm	∼30 mg/mL Human lgG at 2.4 min. residence time
45-500-123	Cytiva 28408260	<4 mL/min.	Each	1.6 cm	∼40 mg/mL Human lgG at 2.4 min. residence time

Description

Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs. MabSelect media are suited for the high flowrates and pressures required when scaling up.

• Oriented coupling of recombinant Protein A ligand to the agarose matrix via an engineered C-terminal cysteine increases IgG binding capacity over other protein A media
• High capacity, low ligand leakage, and high-flow base matrix allow purification of Mabs at process scale
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification
• Designed for use with a syringe, a peristaltic pump, or a chromatography system

MabSelect

• Allows at least five times higher flowrates than conventional cross-linked agarose of similar porosity
• Recombinant Protein A ligand promotes specificity of binding to F_c region of IgG; permits one-step purification

MabSelect Xtra™

• Same recombinant protein A ligand as MabSelect, but with a smaller particle size and greater porosity, for increased dynamic binding capacity
• Allows binding from samples with high expression levels of the monoclonal antibody
• Lowers overall production cost due to the possibility of processing concentrated feedstocks in fewer batches

MabSelect SuRe™ (Superior Resistance)

• Akali-tolerant protein A-derived ligand allows the use of up to 0.5M NaOH for cleaning-in-place (CIP)
• Permits significant cost savings and extension of cycles in regular production

Specifications

• For Use With (Application): For fast purification of monoclonal antibodies from large sample volumes, Protein Purification
• Storage Buffer: 20% Ethanol
• Content And Storage: 4°C to 30°C