Cytiva HiTrap™ IEX HP Prepacked Columns

Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.

Catalog Number	Mfr. No.	Volume (Metric)	Flow Rate	Quantity	Media Type
45-000-191	Cytiva 17115101	1 mL	<4 mL/min.	5 Pk.	SP Sepharose HP
45-000-192	Cytiva 17115201	5 mL	<20 mL/min.	5 Pk.	SP Sepharose HP
45-000-193	Cytiva 17115301	1 mL	<4 mL/min.	5 Pk.	Q Sepharose HP
45-000-194	Cytiva 17115401	5 mL	<20 mL/min.	5 Pk.	Q Sepharose HP
45-000-514	Cytiva 29051324	1 mL	<4 mL/min.	Each	SP Sepharose HP
45-000-515	Cytiva 29051325	1 mL	<4 mL/min.	Each	Q Sepharose HP

Description

 

• Prepacked with Q or SP Sepharose High Performance
• Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series
• Designed for use with syringe, peristaltic pump or chromatography system
• Pressure Max: 3 bar
• 1 and 5mL polypropylene columns

Media:

• Particle size: 24 to 44μm
• pH stability Working Range: 4 to 13

 

Q Sepharose (45-000-193, -194, 515)

• Ligand: Quaternary amine
• Ion Exchanger Type: Strong anion exchanger
• Ionic Capacity: 0.14 to 0.20mmol Cl^-/mL

SP Sepharose (45-000-191, -192, -514) 

• Ligand: Sulphopropyl
• Ion Exchanger Type: Strong cation exchanger
• Ionic Capacity: 0.15 to 0.20mmol H⁺/mL

1mL columns

• Bed Dimensions: 7 x 25mm
• Flow velocity: max 4mL/min.

5mL columns

• Bed Dimensions: 16 x 25mm
• Flow velocity: max 20mL/min.