Gibco™ ExpiCHO™ Expression System Kit

The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells.

Supplier:  Gibco™ A29133

Catalog No. A29133

Includes: 2 vials frozen ExpiCHO-S™ cells, 1 bottle 1000mL ExpiCHO™ Expression Medium, 1 ExpiFectamine™ CHO transfection kit for 1 L of culture, 1 vial Antibody Expressing Positive Control Vector, 1 bottle 100mL OptiPRO™ SFM; store all components at +2° - +8° C, except frozen cells stored in liquid nitrogen

Description

The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium. Transient expression is powered by ExpiFectamine CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed.

All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.

The ExpiCHO Expression System enables:

• Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection
• Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
• Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Use of one-third to one-half less plasmid DNA than other transfection reagents 
• Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
• Scalable expression for culture formats from  <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks

The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.

ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.

All components of the system are animal origin–free.

Specifications

• Type: Expression System
• Delivery Type: Transfection
• Content And Storage: • 2 vials frozen ExpiCHO-S cells
  • 1000 mL ExpiCHO Expression Medium
  • 1 ExpiFectamine CHO Transfection Kit for 1 L of culture
  • 1 vial Antibody Expressing Positive Control Vector
  • 100 mL OptiPRO SFM
  
  Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen.
• Cell Line: CHO-S, SFM Adapted
• Product Line: ExpiCHO™
• Promoter: CMV

• Protein Tag: Untagged
• Quantity: 1 kit
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
• Expression System: Mammalian
• For Use With (Application): Protein Production, Constitutive Expression

For Research Use Only. Not for use in diagnostic procedures.