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Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514
Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.
$343.00 - $459.00
Specifications
Detection Method | Fluorescence |
---|---|
For Use With (Application) | Calcium Assay |
For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
Product Type | Dye |
Emission | 490⁄514 |
Description
- Calcium binding affinity: Kd = 232 nM
- Molecular Weight: ≈1100 d
- Peak Excitation: 490nm
- Peak Emission: 514nm
- Reconstitute in high-quality, freshly opened DMSO
- Recommended concentration: 2 to 5 mM
- Once reconstituted, protect from light and store dessicated at −20°C; avoid freeze-thawing
Specifications
Fluorescence | |
Fluorescence Microscope, Flow Cytometer | |
490⁄514 |
Calcium Assay | |
Dye | |
eBioscience™ |
For Research Use Only. Not for use in diagnostic procedures.
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