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Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Supplier:  Applied Biosystems™ 4337456

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Catalog No. 43-374-56


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Description

Description

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

  • Improve the quality of your results for a wide range of sequencing applications
  • Optimized for long read lengths
  • Better dye mobility characteristics
  • Improved performance reading through GT-rich regions
  • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
  • Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy

With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

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Specifications

Specifications

• 10 tubes of BigDye™ Terminator v3.1 Ready Reaction Mix
• M13 (-21) Primer
• pREF-BDT™ Control DNA
• 1 x 12 mL tube of 5X Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.
SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
BigDye™ Terminator
1000 Reactions
BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), Single Stranded DNA
12-well Strips, 8-well Strip, 96- or 384-well Plate
Ideal for De Novo Sequencing, Resequencing and Finishing with PCR Product, Plasmid, Fosmid and BAC templates
Sequencing Buffer
Fluorescent Dye Terminator Sequencing
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