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Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit
Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.
Supplier: Invitrogen™ 12173011A
Description
Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
Specifications
• 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature • 250 mL PureLink Pro 96 Wash Buffer I; room temperature • 250 mL PureLink Pro 96 Wash Buffer II; room temperature • 75 mL PureLink Pro 96 RNase-free Water; room temperature • 4 PureLink 96-well Total RNA Filter Plates; room temperature • 4 PureLink Pro 96 Elution Plates; room temperature |
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25 min. (After sample homogenization) | |
Bacteria, Cells, Plant, Tissue, Yeast | |
High-throughput Compatible | |
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
Mini | |
Bacteria: ≤109 cells Cells: varies Plant: ≤150 mg Tissue: ≤100 mg Yeast: ≤1.8 mL |
Silica Plate | |
45–170 μL | |
Total RNA | |
Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis | |
4 Plates | |
Room Temperature | |
Up to 33 μg |
Safety and Handling
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For Research Use Only. Not for use in diagnostic procedures.