Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns

Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.

Supplier:  Thermo Scientific™ 063282

Catalog No. DX063282

Description

Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.

Tailored Specificity for IMAC Separation of Proteins

• Retention control by imidazole concentration or pH gradient
• Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
• Effective under native or denaturing HPLC conditions
• Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
• Shipped free from any metal and ready to be charged with the metal of your choice.
• Reusable columns with metal tailored specificity
• Resolve target proteins using a single column in a high resolution gradient run
• High purity separations of metal-binding proteins

Includes: 10μm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.

Specifications

• Column Format: Loading Column
• Flow Rate: 0.1 to 10 mL/min
• Length (Metric): 250 mm
• Mobile Phase Compatibility: pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
• pH: 2 to 12
• Temperature: 60°C
• Packing Material: Spherical, Nonporous Polymer
• Phase: Affinity Separation

• Diameter (Metric): 22 mm
• For Use With: UltiMate 3000 HPLC System
• Max. Pressure: 3000 psi (207 bar)
• Particle Size: 10 μm
• Pore Size: Nonporous
• Stationary Phase: Lysozyne Poly(IDA) Grafts
• Column Type: Separation of Metal-Binding Proteins
• Type: Analytical Column