Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System

Use for fast and easy detection of medium-to-high-target amounts. Cytiva Life Sciences™ Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction

Includes: 45-000-922 and 45-000-923: Labeling reagent; Crosslinker; Control DNA; Blocking agent; ECL detection reagents and ECL Gold hybridization buffer

Catalog Number	Mfr. No.	Includes	Quantity
45-000-925	Cytiva RPN3005	DNA labelling reagent 0.5mL; Glutaraldehyde solution 0.5mL; Water 1.0mL; l Hind III control DNA (10μg/mL) 0.1 ml; Gold hybridization buffer 500mL; Blocking agent 25g	To LabeL 5 μg
45-000-874	Cytiva RPN2105	ECL Detection Reagents 1 and 2, for 4000cm^2 membrane	For 4000 cm2 membrane
45-000-923	Cytiva RPN3001	DNA labeling reagent (2 x 0.5mL); Glutaraldehyde solution (2 x 0.5mL); Water (2 x 1.0mL); l Hind III control DNA 10μg/mL (2 x 0.1mL); Gold hybridization buffer (2 x 500mL); Blocking agent (2 x 25g); ECL detection reagent 1 (2 x 125mL); ECL detection reagent 2 (2 x 125mL)	For Labeling 10μg Nucleic Acid
45-000-924	Cytiva RPN3004	ECL Detection Reagents 1 and 2, for 2000cm^2 membrane	For 2000cm^2 Membrane
45-000-922	Cytiva RPN3000	DNA labeling reagent 0.5mL; Glutaraldehyde solution 0.5mL; Water 1.0mL; l Hind III control DNA (10μg/mL) 0.1mL; Gold hybridization buffer 500mL; Blocking agent 25g; ECL detection reagent 1 125mL; ECL detection reagent 2 125mL	Labels 5 μg Nucleic Acid
45-000-926	Cytiva RPN3006	ECL Gold Hybridization Buffer sufficient for 4000 cm²	Sufficient for 4000 cm^2

Description

 

• • Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL.
  • Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
  • No need to strip blots before reprobing.
  • For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
  • Consistent results combining strong signals with very low backgrounds.

 

 

Safety and Handling

ShelfLife : 3 Months