Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli

Quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Catalog Number	Mfr. No.	No. of Reactions	Vector
K200001	Invitrogen™ K200001	20 Reactions	pCRII
K200040	Invitrogen™ K200040	40 Reactions	pCR2.1

Description

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a 15-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

• Fast and convenient: 15-minute, room-temperature ligation
• Efficient: blue/white screening and >80% clones with correct insert
• Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free: eliminates any enzymatic modifications of PCR product
• Streamlined: does not require use of PCR primers that contain restriction sites
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing

• Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to the 3' ends of PCR products
• Linearized vector supplied in has single 3' deoxythymidine (T) residues
• Allows PCR inserts to ligate efficiently with vector

Cloning, PCR Cloning

Specifications

• Bacterial or Yeast Strain: INVαF ́
• Cloning Method: TA Cloning
• Format: Kit
• Product Line: One Shot™
• Promoter: T7

• Cell Type: Chemically Competent E. coli
• Content And Storage: TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
  
  Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• For Use With (Application): PCR Cloning
• Product Type: Cloning Kit

For Research Use Only. Not for use in diagnostic procedures.