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Edvotek™ Exploring Biotechnology with Green Fluorescent Protein (GFP)
Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP).
Supplier: Edvotek™ 303
Includes: Instructions; BactoBeads; Plasmid DNA for GFP; IPTG; Ampicillin antibiotic; Calcium chloride; ReadyPour™ luria broth agar; Luria broth media for recovery; Petri plates; Pipets; Calibrated transfer pipets; Inoculating loops; Microtest tubes with attached caps; Toothpicks; Dry matrix for columns; Chromatography columns; Green fluorescent protein extracts; Elution buffer; Protein molecular weight standards; Protein denaturation solution; Glycerol solution; Tris-Glycine-SDS buffer; Stains
Requires : Incubation oven; Two water baths; Microwave or hotplate; Automatic micropipet and tips; Pipet pumps or bulbs; Ice; Long-wave UV light; Ring stand and clamps; Lab glassware; Ice; Vertical gel electrophoresis apparatus; Power supply; Three polyacrylamide gels (12%); Plastic trays or large weigh boats for optional staining and destaining; Glacial acetic acid; Methanol
Description
- Bacterial cells are transformed to express the green fluorescent protein (GFP)
- Transformed cells are then grown and the GFP is purified by column chromatography
- The purity of the protein fractions are analyzed by SDS polyacrylamide electrophoresis
Experiment times:
- Module I: Transformation, 45 minutes
- Module II: Isolation of GFP, 45 minutes
- Module III: Purification of GFP by Chromatography, 45 minutes
- Module IV: Analysis by GFP by Denaturing SDS Gel Electrophoresis, one hour
Order Info
Contains materials for six experiments with four modules each.
Recommended Storage
Some components requre freezer storage
Specifications
Green Fluorescent Protein Kit | |
10 experiments | |
Refrigerated. Some components requre freezer storage. |
Provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (gfp) | |
3 hours 15 minutes; Staining time: 30 to 120 minutes |
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