Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.

Catalog Number	Mfr. No.	Conjugate	Excitation/Emission	Quantity
50-927-5	Invitrogen™ 88800574	FITC, Propidium Iodide	488, 535/520, 617	200 Tests
50-927-6	Invitrogen™ 88800672	eFluor 450, 7-AAD	405, 546/450, 647	50 Tests
50-927-7	Invitrogen™ 88800674	eFluor 450, 7-AAD	405, 546/450, 647	200 Tests
509279	Invitrogen™ 88800774	APC, Propidium Iodide	650, 535/660, 617	200 Tests
50-928-0	Invitrogen™ 88800872	PCP-eFluor 710		50 Tests
50-928-1	Invitrogen™ 88800874	PCP-eFluor 710		200 Test
50-929-7	Invitrogen™ BMS500FI100	FITC, Propidium Iodide	488, 535/520, 617	100 Tests
50-929-9	Invitrogen™ BMS500FI20	FITC, Propidium Iodide	488, 535/520, 617	20 Tests
50-930-1	Invitrogen™ BMS500FI300	FITC, Propidium Iodide	488, 535/520, 617	300 Tests
50-246-343	Invitrogen™ 88810272	PE, 7-AAD		50 Tests
50-246-344	Invitrogen™ 88810274	PE, 7-AAD		200 Tests
50-246-345	Invitrogen™ 88810372	PE/Cy7		50 Tests
50-246-346	Invitrogen™ 88810374	PE/Cy7		200 Tests
50-929-4	Invitrogen™ BMS500BT100	Biotin, Propidium Iodide	NA, 535/NA, 617	100 Tests
50-929-5	Invitrogen™ BMS500BT20	Biotin, Propidium Iodide	NA, 535/NA, 617	20 Tests
50-929-6	Invitrogen™ BMS500BT300	Biotin, Propidium Iodide	NA, 535/NA, 617	300 Tests
50-112-8958	Invitrogen™ 88800572	FITC, Propidium Iodide	488, 535/520, 617	50 Tests
50-112-9048	Invitrogen™ 88800772	APC, Propidium Iodide	650, 535/660, 617	50 Tests

Description

Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.

Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

Specifications

• Product Type: Apoptosis Detection Kit