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DYKDDDDK Tag Monoclonal Antibody (FG4R), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA191878

Catalog No. MA191878


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Description

Description

This antibody detects Flag tag from various samples. similar epitope as the FLAG tag from Sigma MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications. The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH. MA1-91878 may be stored at 4oC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20oC, avoiding freeze/thaw cycles.

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
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Specifications

Specifications

DYKDDDDK Tag
Monoclonal
1 mg/mL
PBS with no preservative; pH 7.2
Mouse
Protein A
RUO
Tag
Antibody
IgG2b
Immunocytochemistry, Immunoprecipitation, Western Blot
FG4R
Unconjugated
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Synthetic peptide (DYKDDDDK) coupled to KLH.
100 μg
Primary
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Liquid
Tag
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